Descripción
Human Interleukin 12,IL-12/P40 ELISA KIT
The kit is for the quantitative level of IL-12 in the sample, adopt purified human IL-12 to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to IL-12, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of IL-12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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